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QIAprep& Viral RNA UM Kit
- Viral RNA purification과 real-time PCR을 하나의 kit를 이용하여 실험 가능.
- 간편하고 쉬운 protocol로 1시간 이내 실험 가능.
- UTM, VTM, PBS과 같은 다양한 액체 형태의 transport media에 사용 가능.
- 모든 real-time PCR 장비에 호환 가능.
- Control을 포함한 4-plex (RT)-PCR 가능.
- 2가지 control(inhibition and sampling) 포함.
Overview of the QIAprep& Viral RNA UM workflow
타액과 가글 샘플과의 측정 결과 비교
타액과 가글 샘플과의 측정 결과 비교 Comparison between saliva and gargle samples
Positive high viral titer swab samples spiked into negative saliva and gargle samples. Samples heat-treated at 95°C for 15 min using the QIAprep& workflow for saliva or treated with QIAprep& Buffer AB and incubated at 95°C for 5 min.
바이러스 입자의 검출 한계(LOD) 측정
Reliable assessment of limit of detection (LOD) of viral particles
Limit of Detection (LOD) tested on inactivated SARS-CoV-2 viral particles at 250, 500, 1000, 2000 and 4000 cp/ml. Type of sample diluted in UTM + cell line KG1A at different cp/reaction. All samples tested with KG1A (cell line) amount into the reaction: 1200 cells per reaction. LOD of 8 copies/reaction offers the test sensitivity required.
다양한 transport media를 사용한 샘플 결과 비교
Six transport media compatible with the kit
The kit is compatible with samples collected in non-fixation transport media like UTM, VTM, PBS, 0.9% NaCl, Virocult and eSwab. 8 µl of respective transport medium spiked with indicated amount of IVT + 2 µl Viral RNA UM Prep Buffer + 10 µl Viral RNA UM Master Mix show comparable Ct values.
■ Ordering Information
*제품문의 T. 031-728-3239, E. technical@dawinbiotech.com
#QIAGEN #다윈바이오 #RNA #Viral_RNA #qPCR