연구를 성공으로 이끌 효소를 찾고 계신가요?
QIAGEN은 높은 활성, 일관된 품질, 그리고 신뢰할 수 있는 순도를 갖춘 분자 생물학 및 생명 과학용 효소 라인업을 제공합니다!
최상의 천연 효소, 신규 효소 또는 개량 효소를 생산하도록 설계되었습니다.
특히 임상, 법의학 및 환경 분야의 분석 기기와 어세이(assay) 등 특정 용도를 촉매하는 역할을 수행합니다.
클로닝, 라벨링, 염기서열 분석, 결합, 교환, 절단(nicking) 또는 수리(repairing) 여부에 관계없이 모든 DNA 또는 RNA를 지원합니다.
QIAGEN Enzyme의 특징
1. 효소 순도 및 적격성
QIAGEN 카탈로그의 모든 효소에는 엄격한 품질 관리와 물리적 순도 평가가 적용됩니다.
효소 제품 사양에는 단백질 출처 또는 특성과 단위 정의가 포함됩니다.
품질 관리 보고서에는 일반적으로 효소의 비활성도가 명시되어 있고 분석에는 단위 특성 분석, 단백질 농도 측정(OD280) 및 오염 검사가 포함될 수 있습니다.
제품에 대한 분석 증명서를 요청하고 품질 관리 데이터를 보실 수 있습니다.
2. Lot-to-lot 일관성
시약 로트 간 불일치는 실험 실패와 문제 해결 및 지연으로 인한 시간 낭비를 초래할 수 있습니다. 분자생물학 요구 사항에 맞는 모든 효소를 QIAGEN에 일임하면 당사의 품질 관리 및 품질 보증 실적을 통해 광범위한 인증 및 검증에 필요한 비용을 절감하거나 비용이 발생하지 않을 수 있습니다.
QIAGEN은 독일 힐덴과 미국 베벌리에 ISO 및 GMP 인증 제조 시설을 보유하고 있습니다.
최근 유럽 최고의 효소 제조업체인 BLIRT, S.A.를 인수하면서 생산 능력을 가속화하고 연구 시설을 확장하여 대규모 효소 개발을 위한 기반을 마련했습니다.
제품 정보
Taq DNA Polymerase
동일 정보
- Thermo stable: Yes
- Proof reading: No
- Long range: No
- Resulting ends: 3` dA overhang
- Exonuclease activit: 5` -> 3`
| Product | Cat No. | Description | Features | Applications | Reaction
buffer | Hot
-start |
Taq-B DNA Polymerase
(10,000 U)
| P7250L | PCR amplification of DNA fragments up to 5 kb with minimal optimization | Robust yields, excels at short fragments, GC-rich support | Routine PCR | 10x PCR Buffer I | No |
Taq DNA Polymerase (250U)
| 201203 | Standard and specialized PCR applications | High yield,
>5 kb amplification | Routine PCR, Multiplex PCR,
TA cloning | PCR Buffer,
Q-Solution, CoralLoad Buffer | No |
Phoenix
Hot-Start Taq DNA Polymerase (500 U) | P7590L | Antibody-mediated
hot-start Taq polymerase | Reduced nonspecific amplification,
2 min activation | PCR, qPCR,
RT-qPCR,
Multiplex PCR | Phoenix Hot Start Reaction Buffer, GC Buffer | Antibody |
HotStarTaq
DNA Polymerase
(250 U)
| 203203 | Chemically-mediated
hot-start Taq polymerase | Highest specificity,
15 min activation | Routine PCR, Challenging PCR | HotStarTaq
Buffer Set | Chemical |
AllTaq PCR Core Kit
(250 U)
| 203123 | Antibody-mediated
hot-start polymerase
| Amplifies up to 9 kb,
Gel tracking dyes
RT set up | Routine PCR, Multiplex PCR,
Difficult template | 5x AllTaq PCR Buffer, Q-Solution | Antibody |
Pol I DNA polymerase
| Product | Cat No. | Description | Features | Applications | Exonuclease activity | Resulting ends | Lyo-ready,
glycerol-free |
DNA Polymerase I
(5,000 U)
| P7050L
| Mesophilic E. coli DNA polymerase with both
5’ ➟ 3’ and
3’ ➟ 5’
exonuclease activities
| Synthesizes DNA in
5’ ➟ 3’ direction,
Enables nick-translation | DNA labeling by nick translation, Second-strand cDNA synthesis | 3’ ➟ 5’ 5’ ➟ 3’
| Blunt
| Yes |
Klenow Fragment
(2,500 U)
| P7060L
| Derivative of DNA polymerase I without 5’ ➟ 3’ exonuclease activity
| Synthesizes DNA in
5’ ➟ 3’ direction,
Strand displacement activity | A-tailing for NGS, Blunting of 3’ and 5’ overhangs, Strand displacement, amplification,DNA labeling | 3’ ➟ 5’
| Blunt
| No |
Klenow (3'-5'exo-) Fragment
(10,000 U)
| P7010-LC-L
| Derivative of DNA polymerase I
without 5’ ➟ 3’ and 3’ ➟ 5’
exonuclease activities | Synthesizes DNA in
5’ ➟3’ direction,
Strand displacement activity | A-tailing for NGS, Strand displacement amplification, DNA labeling, Second-strand cDNA synthesis | No proofreading 3’ ➟ 5’ No nick-translation 5’ ➟ 3’ | 3’ dA overhang
| Upon request only |
Other DNA polymerase
| Product | Cat No. | Description | Features | Application | Exonuclease activity | Resulting
ends | Lyo-ready, glycerol-free |
T4 DNA Polymerase
(2,000 U)
| P7080L
| For cloning and DNA labeling by primer extension
| Synthesizes DNA in 5’ ➟ 3’ direction, No strand displacement activity, Powerful exonuclease activity | Blunting 5’ or 3’ overhangs, dsDNA labeling
| Powerful
3’ ➟ 5’
| Blunt | Yes |
T7 DNA Polymerase
(3,500 U)
| P7260L
| Highly processive DNA polymerase with high replication fidelity
| Exceptionally high synthesis rate, Strand displacement activity | Site-specific mutagenesis, Second strand cDNA synthesis, In Situ End Labeling (ISEL) | 3’ ➟ 5’
| Blunt | No |
Terminal Deoxynucleotidyl Transferase (TdT) (6,000 U)
| P7070L
| For extension of blunt 5' overhanging single-stranded DNA
| Adds dNTPs to the 3’-OH terminus of ssDNA or dsDNA
| Homopolymeric tailing to 3’-OH, TUNEL assay, 5’-RACE, DNA labeling of 3'-ends | No | 3` dA
overhang | No |
RNA polymerase
| Product | Cat No. | Description | Features | Applications | Lyo-ready, glycerol-free |
Poly(A) Polymerase
(1,000 U)
|
P7460L
| For making poly(A) tailed RNA and 3’ end-labeling of RNA
| Template independent catalysis of the addition of AMP from ATP to the 3’-OH of RNA
| 3’ end-labeling of RNA, Applications in mRNA therapeutics | No |
T7 RNA Polymerase
(50,000 U)
| P7180L
| For in vitro transcription and synthesis of RNA from a DNA template | Synthesizes RNA in 5’ ➟ 3 direction of DNA template, High specificity to T7 promoter, Catalyzes Mg2+-dependent synthesis of RNA from rNTPs | RNA probe preparation, Applications in mRNA therapeutics
| No |
Reverse transcriptases and DNA synthesis
| Product | Cat No. | Description | Features | Application | Long
range | Lyo-ready, glycerol-free |
EnzScript Reverse Transcriptase
(10,000 U)
| P7600L
| For reverse transcription from RNA for first strand cDNA synthesis and RT-PCR
| No RNase H activity, cDNA transcripts >5 kb, Reaction temperature up to 50°C, Template switching | cDNA synthesis, One-step RT-PCR, Template switching-based adapter addition for RNA sequencing | Yes | Yes |
StableScript Reverse Transcriptase
(250 U)
| P7720L
| For reverse transcription of long transcripts, inhibited samples and difficult templates with secondary structures
| No RNase H activity, cDNA transcripts up to 12.3 kb, Superior thermostability
(up to 65°C), Increased inhibitor tolerance | cDNA synthesis, One-step RT-qPCR, Long range RT-PCR, Ligation-based adapter addition for RNA sequencing | Yes | Yes |
RNase Inhibitor
(20,000 U)
| Y9240L
| For use where the presence of RNases may pose a risk to RNA quality
| Non-competitive inhibitor of pancreatic-type ribonucleases, No influence on polymerase or reverse transcriptase activity | cDNA synthesis, One-step RT-PCR | - | Yes |
RNase Inhibitor Hu (2,000 U)
| RT35-020
| Maximum RNA protection where intact RNA is required
| High thermostability and resistance to oxidative agents, Stable at 37°C for 4 weeks, Completely inhibits pancreatic-type ribonucleases, No influence on polymerase or reverse transcriptase activity, DNase and RNase free | In
cDNA synthesis, In RT-PCR and RT-qPCR, In in vitro transcription and translation | - | Upon request only |
RNase H (5,000 U)
| Y9220L
| Cleaves the RNA strand of DNA:RNA hybrids
| Does not degrade ssRNA or dsRNA
| Removal of mRNA during, second-strand cDNA synthesis | - | Yes |
RT-PCR
동일정보
- Real-time PCR: Yes
- Multiplex PCR: Yes-
- Application: Gene expression analysis
| Product | Cat No. | Description | Features | Hot-start |
QuantiTect
Probe RT-PCR Kit
(200 rxn) | 202443 | One-step RT-qPCR using
sequence-specific probes | Sensitive detection, dUTP/UNG system | Chemical |
QuantiTect
Multiplex RT-PCR Kit
(200 rxn)
| 204643 | One-step RT-qPCR using
SYBR Green | Multiplex five targets, dUTP/UNG | Chemical |
ZipScrip
One-Step RT-qPCR Kit
(1,000 rxn)
| P7640L | Highly sensitive RT-qPCR kit | Detects 0.1 pg RNA, inhibitor resistant | Antibody |
Probe PCR
동일정보
- Multiplex PCR: Yes
- Hot-start: Antibody
| Product | Cat No. | Description | Features | Applications | Real-time PCR | End-point PCR |
QuantiNova
Probe PCR Kit
(100 rxn)
| 208252 | Fast probe-based real-time PCR | Rare target detection; room temperature setup | Gene expression,
gDNA analysis | Yes | No |
| QuantiNova Multiplex PCR Kit (100 rxn) | 208452 | Fast multiplex
real-time PCR | Detects five targets; visual pipetting control | Multiplex gene expression | Yes | No |
| UltraRun LongRange PCR Kit (100 rxn) | 206442 | Ultra-long-range PCR | Amplification up to 40 kb; low error rate | Cloning, sequencing | No | Yes |
UCP Probe PCR Kit
(500 rxn)
| 208214 | Ultra-clean
probe PCR | No residual DNA background; inhibitor robust | Microbial testing,
NGS prep | Yes | No |
DNA Ligase
| Product | Cat No. | Descripiton | Features | Application | Lyo-ready, glycerol-free |
WGS Ligase
(24 rxn)
| L6030-W-L | Optimized for ligation following WGS Fragmentation in NGS library construction
| Joins blunt and cohesive ends, Repairs single-stranded nicks in duplex DNA, RNA, or DNA-RNA hybrid
| NGS library construction
| Upon request only |
T4
DNA Ligase
(150,000 U)
| L6030-LC-L
| Industry standard ligase
| Joins blunt and cohesive ends, Seals nicks in duplex DNA, RNA or DNA-RNA hybrid
| Restriction cloning, TA-cloning, Adapter ligation
| Yes |
T7
DNA Ligase
(900,000 U)
| L6020L | For ligating cohesive ends of duplex DNA
| Only efficiently joins cohesive-end termini, Low efficiency joining blunt ends, Repairs single-stranded nicks in duplex DNA
| Highly efficient for cohesive end ligation
| No |
T3
DNA Ligase
(900,000 U) | L6010L | ATP-dependent dsDNA ligase with increased salt tolerance
| Joins blunt and cohesive ends, Repairs single stranded nicks in duplex DNA, ATP dependent, Increased salt tolerance | Ligating dsDNA in high ionic strength NaCl (1.0 M)
| No |
E. coli
DNA Ligase
(2,500 U)
| L6090L | For ligation of DNA at nicks and cohesive termini
| Does not ligate blunt ends, NAD+ dependent | cDNA cloning by replacement synthesis
| No |
Taq
DNA Ligase
(20,000 U)
| L6060L | Thermostable ligase for joining or ligation of DNA fragments
| Thermostable, Seals nicks, Discriminates against mismatch ligation, NAD+ dependent | Ligase Chain Reaction (LCR), Ligase Detection Reaction (LDR), High temperature ligation
| Upon request only |
Tth
DNA Ligase
(250 U)
| EN13-025 | Thermostable NAD+-dependent dsDNA ligase
| High thermostability, High specificity for SNP detection, Not active against ssDNA or RNA and blunt-ended DNA | Ligase Chain Reaction (LCR), Ligase Detection Reaction (LDR), NGS sequencing, Repeat expansion detection, Rolling circle amplification (RCA), Proximity ligation assay (PLA)
| No |
*제품문의 T. 031-728-3239, E. technical@dawinbiotech.com
#QIAGEN #다윈바이오 #Enzyme #PCR #RT_PCR #Probe
연구를 성공으로 이끌 효소를 찾고 계신가요?
QIAGEN은 높은 활성, 일관된 품질, 그리고 신뢰할 수 있는 순도를 갖춘 분자 생물학 및 생명 과학용 효소 라인업을 제공합니다!
최상의 천연 효소, 신규 효소 또는 개량 효소를 생산하도록 설계되었습니다.
특히 임상, 법의학 및 환경 분야의 분석 기기와 어세이(assay) 등 특정 용도를 촉매하는 역할을 수행합니다.
클로닝, 라벨링, 염기서열 분석, 결합, 교환, 절단(nicking) 또는 수리(repairing) 여부에 관계없이 모든 DNA 또는 RNA를 지원합니다.
QIAGEN Enzyme의 특징
1. 효소 순도 및 적격성
QIAGEN 카탈로그의 모든 효소에는 엄격한 품질 관리와 물리적 순도 평가가 적용됩니다.
효소 제품 사양에는 단백질 출처 또는 특성과 단위 정의가 포함됩니다.
품질 관리 보고서에는 일반적으로 효소의 비활성도가 명시되어 있고 분석에는 단위 특성 분석, 단백질 농도 측정(OD280) 및 오염 검사가 포함될 수 있습니다.
제품에 대한 분석 증명서를 요청하고 품질 관리 데이터를 보실 수 있습니다.
2. Lot-to-lot 일관성
시약 로트 간 불일치는 실험 실패와 문제 해결 및 지연으로 인한 시간 낭비를 초래할 수 있습니다. 분자생물학 요구 사항에 맞는 모든 효소를 QIAGEN에 일임하면 당사의 품질 관리 및 품질 보증 실적을 통해 광범위한 인증 및 검증에 필요한 비용을 절감하거나 비용이 발생하지 않을 수 있습니다.
QIAGEN은 독일 힐덴과 미국 베벌리에 ISO 및 GMP 인증 제조 시설을 보유하고 있습니다.
최근 유럽 최고의 효소 제조업체인 BLIRT, S.A.를 인수하면서 생산 능력을 가속화하고 연구 시설을 확장하여 대규모 효소 개발을 위한 기반을 마련했습니다.
제품 정보
Taq DNA Polymerase
동일 정보
- Thermo stable: Yes
- Proof reading: No
- Long range: No
- Resulting ends: 3` dA overhang
- Exonuclease activit: 5` -> 3`
buffer
-start
(10,000 U)
>5 kb amplification
TA cloning
Q-Solution, CoralLoad Buffer
Hot-Start Taq DNA Polymerase (500 U)
hot-start Taq polymerase
2 min activation
RT-qPCR,
Multiplex PCR
DNA Polymerase
(250 U)
hot-start Taq polymerase
15 min activation
Buffer Set
(250 U)
hot-start polymerase
Amplifies up to 9 kb,
Gel tracking dyes
RT set up
Difficult template
Pol I DNA polymerase
glycerol-free
(5,000 U)
Mesophilic E. coli DNA polymerase with both
5’ ➟ 3’ and
3’ ➟ 5’
exonuclease activities
Synthesizes DNA in
5’ ➟ 3’ direction,
Enables nick-translation
DNA labeling by nick translation, Second-strand cDNA synthesis
3’ ➟ 5’
5’ ➟ 3’
(2,500 U)
Synthesizes DNA in
5’ ➟ 3’ direction,
Strand displacement activity
A-tailing for NGS, Blunting of 3’ and 5’ overhangs, Strand displacement, amplification,DNA labeling
(10,000 U)
Derivative of DNA polymerase I
without 5’ ➟ 3’ and 3’ ➟ 5’
exonuclease activities
Synthesizes DNA in
5’ ➟3’ direction,
Strand displacement activity
A-tailing for NGS, Strand displacement amplification, DNA labeling, Second-strand cDNA synthesis
No proofreading 3’ ➟ 5’
No nick-translation 5’ ➟ 3’
Other DNA polymerase
ends
(2,000 U)
For cloning and DNA labeling by primer extension
Synthesizes DNA in 5’ ➟ 3’ direction, No strand displacement activity, Powerful exonuclease activity
Blunting 5’ or 3’ overhangs, dsDNA labeling
3’ ➟ 5’
(3,500 U)
Exceptionally high synthesis rate, Strand displacement activity
Site-specific mutagenesis, Second strand cDNA synthesis, In Situ End Labeling (ISEL)
Homopolymeric tailing to 3’-OH, TUNEL assay, 5’-RACE, DNA labeling of 3'-ends
overhang
RNA polymerase
(1,000 U)
P7460L
3’ end-labeling of RNA, Applications in mRNA therapeutics
T7 RNA Polymerase
(50,000 U)
Synthesizes RNA in 5’ ➟ 3 direction of DNA template, High specificity to T7 promoter, Catalyzes Mg2+-dependent synthesis of RNA from rNTPs
RNA probe preparation, Applications in mRNA therapeutics
Reverse transcriptases and DNA synthesis
range
(10,000 U)
No RNase H activity, cDNA transcripts >5 kb, Reaction temperature up to 50°C, Template switching
cDNA synthesis, One-step RT-PCR, Template switching-based adapter addition for RNA sequencing
(250 U)
No RNase H activity, cDNA transcripts up to 12.3 kb, Superior thermostability
(up to 65°C), Increased inhibitor tolerance
cDNA synthesis, One-step RT-qPCR, Long range RT-PCR, Ligation-based adapter addition for RNA sequencing
(20,000 U)
Non-competitive inhibitor of pancreatic-type ribonucleases, No influence on polymerase or reverse transcriptase activity
cDNA synthesis, One-step RT-PCR
High thermostability and resistance to oxidative agents, Stable at 37°C for 4 weeks, Completely inhibits pancreatic-type ribonucleases, No influence on polymerase or reverse transcriptase activity, DNase and RNase free
In
cDNA synthesis, In RT-PCR and RT-qPCR, In in vitro transcription and
translation
Removal of mRNA during, second-strand cDNA synthesis
RT-PCR
동일정보
- Real-time PCR: Yes
- Multiplex PCR: Yes-
- Application: Gene expression analysis
Probe RT-PCR Kit
(200 rxn)
sequence-specific probes
Multiplex RT-PCR Kit
(200 rxn)
SYBR Green
One-Step RT-qPCR Kit
(1,000 rxn)
Probe PCR
동일정보
- Multiplex PCR: Yes
- Hot-start: Antibody
Probe PCR Kit
(100 rxn)
gDNA analysis
real-time PCR
(500 rxn)
probe PCR
NGS prep
DNA Ligase
(24 rxn)
Joins blunt and cohesive ends, Repairs single-stranded nicks in duplex DNA, RNA, or DNA-RNA hybrid
DNA Ligase
(150,000 U)
Joins blunt and cohesive ends, Seals nicks in duplex DNA, RNA or DNA-RNA hybrid
Restriction cloning, TA-cloning, Adapter ligation
DNA Ligase
(900,000 U)
Only efficiently joins cohesive-end termini, Low efficiency joining blunt ends, Repairs single-stranded nicks in duplex DNA
Highly efficient for cohesive end ligation
T3
DNA Ligase
(900,000 U)
Joins blunt and cohesive ends, Repairs single stranded nicks in duplex DNA, ATP dependent, Increased salt tolerance
Ligating dsDNA in high ionic strength NaCl (1.0 M)
DNA Ligase
(2,500 U)
Does not ligate blunt ends, NAD+ dependent
DNA Ligase
(20,000 U)
Thermostable, Seals nicks, Discriminates against mismatch ligation, NAD+ dependent
Ligase Chain Reaction (LCR), Ligase Detection Reaction (LDR), High temperature ligation
DNA Ligase
(250 U)
High thermostability, High specificity for SNP detection, Not active against ssDNA or RNA and blunt-ended DNA
Ligase Chain Reaction (LCR), Ligase Detection Reaction (LDR), NGS sequencing, Repeat expansion detection, Rolling circle amplification (RCA), Proximity ligation assay (PLA)
*제품문의 T. 031-728-3239, E. technical@dawinbiotech.com
#QIAGEN #다윈바이오 #Enzyme #PCR #RT_PCR #Probe