Genomics Workflow 제품 선택 : Merck/Sigma 제품 소개

Molecular Cloning, Expression

Cloning, Expression

Cat.No.	Description	Unit	List price
69864-3CN	pET-28a(+) DNA - Novagen	10 μg	429,500
71146-3	pETDuet-1 DNA - Novagen	10 μg	677,100
TR-1003-G	Polybrene Transfection Reagent	1 mL	85,800
L2542-500ML	LB Broth (Miller)	500 mL	64,900
M6030-1KG	M9, Minimal Salts, 5X	1 kg	491,100
71754-3	Terrific Broth - Novagen	500 g	164,500
S1797-100ML	SOC Medium	100 mL	289,500
Y1625-1KG	Yeast Extract	1 kg	547,700
L3022-1KG	LB Broth (Lennox)	1 kg	289,500

Transformation - Competent Cells

Cat.No.	Description	Unit	List price
69450-4CN	BL21(DE3) Competent Cells - Novagen	1 mL	407,700
69451-4CN	BL21(DE3)pLysS Competent Cells - Novagen	1 mL	427,500

Transfection

• Eukaryotic cells, insect cells에 Transfection 최적화
Ex>Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080,

K-562, RAW 264.7, Jurkat, PC-12, HCT 116

Cat.No.	Description	Unit	List price
6366546001	X-tremeGENE™ HP DNA Transfection Reagent	5 x 1 mL	3,424,200
6365809001	X-tremeGENE™ 9 DNA Transfection Reagent	5 x 1 mL	3,163,000
70967-3CN	GeneJuice® Transfection Reagent	1 mL	740,200

DNA, RNA Sample Preparation

GenElute™ - E Single Spin Technology

• Negative chromatography 방법을 이용하여 단 한 번의 centrifuge로
원하는 nucleic acid 추출 가능

• Ethanol이 들어간 washing buffer를 사용하지 않아 PCR contaminant가 포함될 우려가 없음

Cat.No.	Description	Unit	List price
T9424-200ML	TRI Reagent®	200 mL	807,300
R0901-500ML	RNAlater®	500 mL	1,068,800
P2069-400ML	Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA	400 mL	645,900
EC600-250RXN	GenElute™-E DNA Cleanup Kit	250 reactions	1,397,200
EC300-250RXN	GenElute™-E Single Spin Tissue DNA Kit	250 reactions	1,824,000
P2308-100MG	Proteinase K from Tritirachium album	100 mg	338,800
EC100-250RXN	GenElute™-E Single Spin Blood DNA Kit	250 reactions	1,522,400
11667327001	DNA Isolation Kit for Mammalian Blood	25 reactions	345,600
11814770001	DNA Isolation Kit for Cells and Tissues	10 isolations	362,300
NA0300-1KT	GenElute™ HP Plasmid Maxiprep Kit	10 purifications	405,400
NA9604-1KT	GenElute™ HP 96-Well Plasmid Miniprep Kit	4 x 96 well plates	1,902,900
EC800-250RXN	GenElute™-E RNA Cleanup Kit	250 reactions	1,539,600
EC111-100ML	GenElute™-E Tissue Stabilizer	100 mL	258,700

Gene Amplification

Cat.No.	Description	Unit	List price
71975-3	KOD Xtreme Hot Start DNA Polymerase	200 units	792,200
71087-4CN	KOD XL DNA Polymerase	1250 units	2,570,100
D8187-2.5KU	JumpStart™ REDTaq® DNA Polymerase	2500 units	3,840,800
R2523-100RXN	REDTaq® ReadyMix™ PCR Reaction Mix	100 reactions	371,600
P2893-400RXN	JumpStart™ Taq ReadyMix™	400 reactions	1,233,200
KK4602	KAPA SYBR® FAST, Universial	10 mL	569,100
KK4605	KAPA SYBR® FAST, ABI PRISM	10 mL	569,100
KK4611	KAPA SYBR® FAST, LightCycler®480	10 mL	569,100
12140306001	GC-RICH PCR System	50 reactions	462,500
4913949001	FastStart Universal Probe Master (Rox)	250 x 20 μL	190,000
3531287001	Transcriptor Reverse Transcriptase	200 reactions	1,418,900
M1302-40KU	M-MLV Reverse Transcriptase	4000 units	746,700
5893151001	Transcriptor Universal cDNA Master	100 reactions	1,695,500
11483188001	First Strand cDNA Synthesis Kit for RT-PCR (AMV)	30 reactions	758,200
B0300-5VL	Betaine solution, 5 M	5 vials	213,700
AMPD1-1KT	DNase I	1 kit	230,200
M8662-5VL	Mineral oil	5 vials	219,300

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SigmaGenomics Workflow 제품 선택