Western Blot Workflow : Merck/Sigma 제품 소개

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조회수 1230

Protein Extraction, Sample Preparation

Lysis, Extraction
RIPA : Mammalian cell lysis, protein-protein interaction 연구 목적 사용 가능
BugBuster^® : Sonication 없이 E. coli의 soluble protein 추출
CHAPS : Receptor 또는 membrane protein 추출
Lysozyme : Bacteria protein 추출

Cat.No.	Description	Unit	List price (원)
20-188	RIPA Lysis Buffer 10 X 100 mL	1 L	177,900
71456-3	BugBuster^® Master Mix	500 mL	400,500
C9426-5G	CHAPS hydrate, BioReagent, ≥98% (TLC)	5 g	568,200
10837059001	Lysozyme	10 g	336,200



Protease Inhibitors
Animals, plants, yeast, bacteria, fungi 등 대부분의 조직에서 추출 시 proteolytic activity을 inhibition
Serine proteases, cysteine proteases, metalloproteases 등 다양한 protease inhibition

Cat.No.	Description	Unit	List price (원)
4906837001	PhosSTOP™, 20 tablets	20 tablets	411,800
11873580001	cOmplete™, EDTA-free Protease Inhibitor Cocktail	20 tablets	584,300
11524488001	Pepstatin A, 50 mg	50 mg	541,500
P2714-1BTL	Protease Inhibitor Cocktail, for general use, lyophilized powder	1 bottle	147,700


Buffer Exchange, Concentration
Fast- Single spin으로 빠르게 buffer exchange
Gentle- 독특한 구성으로 지속적인 buffer exchange
Less buffer- 1.5 mL의 적은 buffer만 필요

Cat.No.	Description	Unit	List price (원)
UFC501024	Amicon^® Ultra – 0.5 mL Filters, 24 pack	24 pkg	206,200
UFC201024	Amicon^® Ultra – 2 mL Filters, 24 pack	24 pkg	261,100
UFC801024	Amicon^® Ultra – 4 mL Filters, 24 pack	24 pkg	336,100
UFC901024	Amicon^® Ultra – 15 mL Filters, 24 pack	24 pkg	557,200


Electrophoresis


Electrophoresis Reagent, Buffer

Cat.No.	Description	Unit	List price (원)
LUXRGTKIT	mPAGE^® Lux Bis-Tris Reagent Kit	1 kt	383,000
94964	BLUeye Prestained Protein Ladder	500 μL	299,500
MPSTD4	mPAGE^® Color Protein Standard	500 μL	243,000
MPSTD3	mPAGE^® Unstained Protein Standard	500 μL	176,000
MPSTD2	mPAGE^® Western Protein Standard	250 μL	150,500
MPMES	mPAGE^® MES SDS Running Buffer Powder	pack of 5 ea	54,400
MPM0PS	mPAGE^® MOPS SDS Running Buffer Powder	pack of 5 ea	47,700
MPSB-10ML	mPAGE™ 4X LDS Sample Buffer	10 mL	23,800
A3678-25G	Ammonium persulfate (APS) for electrophoresis, ≥98%	25 g	75,100
T22500-5ML	N,N,N′,N′-Tetramethylethylenediamine (TEMED) ReagentPlus^®, 99%	5 mL	52,600
G8898-500G	Glycine, ≥99%	500 g	144,500
T5816-100G	Tricine, cell culture tested, ≥99%	100 g	266,100
RDD008-1KG	Trizma^® base, anhydrous, free-flowing, Redi-Dri™, ≥99.9%	1 kg	240,700
L3771-1KG	Sodium dodecyl sulfate ≥98.5%	1 kg	981,400
M3148-25ML	2-Mercaptoethanol suitable for cell culture, 99%	25 mL	48,800
D9163-5G	dl-Dithiothreitol (DTT), ≥99%	5 g	340,300

Precast Protein Gels

mPAGE™ Precast Gel
기존 electrophoresis tanks에 호환가능	합리적인 가격
중성 pH로 단백질 변형 방지

Cat.No.	Description	List price (원)
MP41G10	mPAGE™ 4-12% Bis-Tris, 10x8, 10-well	157,500
MP41G12	mPAGE™ 4-12% Bis-Tris, 10x8, 12-well	157,700
MP41G15	mPAGE™ 4-12% Bis-Tris, 10x8, 15-well	157,500
MP42G10	mPAGE™ 4-20% Bis-Tris, 10x8, 10-well	157,500
MP42G12	mPAGE™ 4-20% Bis-Tris, 10x8, 12-well	157,700
MP42G15	mPAGE™ 4-20% Bis-Tris, 10x8, 15-well	157,700
MP81G10	mPAGE™ 8-16% Bis-Tris, 10x8, 10-well	157,500
MP81G12	mPAGE™ 8-16% Bis-Tris, 10x8, 12-well	157,500
MP81G15	mPAGE™ 8-16% Bis-Tris, 10x8, 15-well	157,500

Protein Transfer

Transfer Membrane, Blotting Papers
Membrane에 protein의 높은 흡착력으로 강한 단백질 signal
더 긴 유통기한	쉽게 잘라서 사용 가능

Cat.No.	Description	List price (원)
ISEQ85R	Immobilon^®-P^SQ PVDF Transfer Membrane, 0.2 μm	607,300
IPVH85R	Immobilon^®-P PVDF Transfer Membrane, 0.45 μm	607,300
HATF85R	Immobilon^®-NC Transfer Membrane, 0.45 μm	486,800
IMDISP	Immobilon^® NOW Dispenser for 8.5 x 10 cm rolls	511,700

Blocking, Antibody Incubation

Loading Control Ab
모든 well에서 로딩된 샘플을 정량화하여 내부 표준으로 활용
각 well마다 단백질 양을 확인하여 통해 관찰 대상의 변화를 설명할 수 있음

Cat.No.	Description	Unit	List price (원)
SAB2108668-100UL	Anti-GAPDH, polyclonal	100 μL	807,500
A2066-100UL	Anti-Actin, polyclonal	100 μL	688,600
SAB4200714-100UL	Anti-HSP70	100 μL	776,000
MAB374	Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, Clone 6C5	200 μg	568,800
A5441-.2ML	Anti-β-Actin antibody, Clone AC-15	200 μL	918,300
A2066-100UL	Anti-Actin antibody, Rabbit polyclonal	100 μL	688,600
CB1001-500UG	Anti-GAPDH Mouse mAb (6C5)	500 μg	675,000
G8795-100UL	Anti-GAPDH antibody, Clone GAPDH-71.1	100 μL	868,800
G9545-200UL	Anti-GAPDH antibody, Rabbit polyclonal	200 μL	1,123,700
AB10526	Anti-COX4 (Cytochrome C Oxidase), rabbit polyclonal	100 μg	661,500
07-371	Anti-Histone H2B Antibody	200 μg	741,200
05-1352	Anti-Histone H2B Antibody, Clone 5HH2-2A8	100 μL	786,100
MABE288	Anti-PCNA Antibody, Clone PC10	200 μL	668,200
ABS16	Anti-GAPDH Antibody	100 μg	542,400
AB2302	Anti-GAPDH Antibody	100 μg	661,500

머크의 모든 항체를 한눈에!
머크/씨그마알드리치의 9만종 포트폴리오를 검색하고 실시간 업데이트 되는 할인 및 이벤트 정보를 확인하세요.



Immunodetection Reagent and Consumable
가장 빠른 면역 검출	항체-항원 결합 증가	낮은 Background

Cat.No.	Description	Unit	List price (원)
SNAP2BHMN0100	SNAP i.d.^® 2.0 Consumables, Mini Blot Holders		541,900
SNAPABTR	SNAP i.d.^® 2.0 Antibody Collection Tray		48,700
SNAP2RL	SNAP i.d.^® 2.0 Blot Roller		51,600
SNAP2BHMB050	SNAP i.d.^® 2.0 MultiBlot Holders		455,000
SNAP2FRMN01	SNAP i.d.^® 2.0 Mini Blot Holding Frame (single pack)		227,900
WBAVDCH01	Immobilon^® Block-CH Reagent, Chemiluminescence Detection	500 mL	224,800
WBAVDP001	Immobilon^® Block-PO Reagent, Phosphoprotein Detection	500 mL	269,500
T6789-10PAK	Tris-Buffered Saline, with BSA, pH 8.0, powder	10 pack	527,100
91414-10TAB	Tris-Buffered Saline with Tween® 20 detergent, tablets pH 7.6	10 tablet	281,000
T6664-10PAK	Tris-Buffered Saline, pH 8.0, powder	10 pack	134,200
P9416-100ML	Tween^® 20 detergent	100 mL	91,600
WBLUF0100	Immobilon^® Forte Western HRP Substrate	100 mL	241,400
WBLUR0100	Immobilon^® Crescendo Western HRP Substrate	100 mL	192,600
WBKLS0100	Immobilon^® Western Chemiluminescent HRP Substrate	2 x 50 mL	205,000
WBULS0100	Immobilon^® ECL Ultra Western HRP Substrate	2 x 50 mL	321,800
407207-1KITCN	SignalBoost™ Immunoreaction Enhancer Kit		453,200
T9455-100ML	3,3′,5,5′-Tetramethylbenzidine (TMB), Enhanced HRP Membrane Substrate	100 mL	206,200
2504	ReBlot™ Plus Strong Antibody Stripping Solution	10 x 50 mL	211,600

* 관련문의: Tel. 031-728-3291 E-mail. dawinbio@dawinbio.com

#Merck#sigma#Buffer#WesternBlot#SDS-PAGE#Transfer#RIPAbuffer

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