Cell Culture Workflow : Merck/Sigma 제품 소개

알림

조회수 1562

Prepare

Cell

Stem Cell	Primary Cell	Mammalian Cell Line
ES (Embryonic Stem Cell) 및 IPSC (Induced Pluripotent Stem Cell) 뿐만 아니라 Stem Cell의 분화 및 성숙을 위한 미디어를 제공합니다.	조직에서 파생된 Primary Cell로써, Drug Screening 및 ADME/Tox 연구에 필수적인 혈액, 환자 종양, 간세포 등을 제공합니다.	질병 및 생물학적 시스템 모델링에 필수적이며 단백질, 항체, 바이러스 및 백신 생산 등의 목적으로 사용될 수 있는 인간 및 기타 포유류 종의 다양한 Cell Line을 ECACC와 협럭하여 제공합니다.



Bottle Top Filter
Media, FBS 사용 전 Filtration으로 오염 방지
Millipore^®브랜드의 고품질 멸균필터 사용

Cat.No.	Description	Unit	List Price (원)
S2GPU11RE	Millipore^® Stericup^® Vacuum Filtration System, pore size 0.22 μm, polyethersulfone membrane, funnel capacity 1000 mL, pack of 12 ea	100 mL	862,600
S2GPU05RE	Millipore^® Stericup^® Vacuum Filtration System, pore size 0.22 μm, polyethersulfone membrane, funnel capacity 500 mL, pack of 12 ea	100 mL	832,600



Cell Freezing
DMSO : 가장 널리 사용되는 Sigma-Aldrich® 브랜드의 Cryotectant
CryoStor^®: T-cell, hiPSC 등에 최적화된 Cryopreservation media

Cat.No.	Description	Unit	List Price (원)
C2874-100ML	CryoStor^® Cell Cryopreservation Media, 10% DMSO	100 mL	862,600
C2999-100ML	CryoStor^® Cell Cryopreservation Media, 5% DMSO	100 mL	832,600
C9249-100ML	CryoSOfree™ DMSO-free Cryopreservation Medium	100 mL	679,500
D2650-100ML	Dimethyl sulfoxide, Hybri-Max™, Sterile-filtered, BioReagent, Suitable for hybridoma, ≥99.7%	100 mL	451,000

Grow

Cell Dissociation
Trypsin - EDTA : Adherent cells을 dissociation하는 데 가장 일반적으로 사용
StableCell™ : 실온보관 가능, GMP에서 생산
Accutase^® : iPSC, glioblastoma cells 등의 nonadherent cells을 dissociation
Accumax™ : CHO, BHK, 293, COS, Sf9, Hybridoma 등의 suspension cells을 dissociation

Cat.No.	Description	Unit	List Price (원)
T2601-100ML	StableCell™ Trypsin Solution	100 mL	28,500
A7089-100ML	Accumax™ Solution	100 mL	293,500
A6964-100ML	Accutase^® Solution	100 mL	111,000

Organoid
오가노이드는 자가 복제 및 자기 조직이 가능하고 장기 기능을 보여 주는 primary cell 또는 stem cell에서 유래한 체외 유래 3D 세포 군집체로써, 아래 3가지 특징으로 기존 모델 시스템의 한계를 해결합니다.
1차 세포와 유사한 조성 및 구성
생체 내 조건의 관련 모델
장기 배양을 위한 안정적 시스템

Cat.No.	Description	Unit	List Price (원)
SCM162	DMEM/F-12 PLUS Basal Medium	500 mL	129,800
SCM300	3dGRO™ Organoid Dissociation Reagent	100 mL	94,200
SCM301	3dGRO™ Organoid Freeze Medium	50 mL	317,500
SCC111	R-Spondin1-expressing 293T Cell Line	≥1 X 10⁶ cells/vial	853,500
SCM104	3dGRO™ R-Spondin 1 Conditioned Media Supplement	50 mL	399,400
SCM105	3dGRO™ L-WRN Conditioned Media Supplement	50 mL	442,300


FBS
안정적인 공급	35년 이상 일관된 품질
합리적인 가격	Gamma-irradiation premier serum
다양한 Origin	Hybridoma, Stem cell에 최적한 serum

Cat.No.	Description	Unit	List Price (원)
TMS-013-BKR	Fetal Bovine Serum : US origin, suitable for cell culture	500 mL	1,492,700
F2442-500ML	Fetal Bovine Serum : US origin, suitable for cell culture, suitable for hybridoma	500 mL	1,091,500
ES-009-B	Fetal Bovine Serum, US origin, EmbryoMax ES Cell Qualified FBS, sterile-filtered, suitable for stem cell culture	500 mL	1,656,800
12107C-500ML	Fetal Bovine Serum, USA origin, γ-irradiated by SER-TAIN process, sterile-filtered, suitable for cell culture	500 mL	1,875,600
12003C-500ML	Fetal Bovine Serum, Australia origin, USDA approved, sterile-filtered, suitable for cell culture	500 mL	2,313,300


Media
세포 배양 애플리케이션에 최적의 제품으로, 자사의 최첨단 시설에서 제조
다양한 포유류 세포 배양을 위한 생리학적 환경에 맞는 여러 종류의 배지를 보유
분말상 및 액상 보유

Cat.No.	Description	Unit	List Price (원)
D5796-500ML	DMEM- High glucose medium with no added factor	500 mL	15,600
D6046-500ML	DMEM- Low glucose	500 mL	15,800
D6429-500ML	DMEM- High glucose medium with sodium pyruvate added	500 mL	16,400
R0883-500ML	RPMI-1640 Medium with sodium bicarbonate	500 mL	15,100
R8758-500ML	RPMI-1640 Medium with L-glutamine	500 mL	20,300
M4530-500ML	Medium 199, with Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered	500 mL	15,800
M4655-500ML	Minimum Essential Medium Eagle, with Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered	500 mL	15,800
D8437-500ML	DMEM F12, with L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered	500 mL	25,800


Buffer
초고순도 substances
수천 편의 논문 인용 횟수
가장 널리 사용되는 Sigma-Aldrich^® 브랜드의 DPBS

Cat.No.	Description	Unit	List Price (원)
D8537-1L	Dulbecco's Phosphate Buffered Saline- 1 L	1 L	59,300
D8537-500ML	Dulbecco's Phosphate Buffered Saline- 500 mL	500 mL	32,500
H8264-500ML	Hanks′ Balanced Salt Solution, Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture	500 mL	38,900
H6648-500ML	Hanks' Balanced Salt Solution, Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture	500 mL	40,200
H23830-500G	HEPES, reagent grade, ≥99% (titration), crystalline	500 g	367,000
H4034-1KG	HEPES, BioPerformance Certified, >=99.5% (titration), cell culture tested	1 kg	1,491,300
H3784-1KG	HEPES sodium salt, BioPerformance Certified, suitable for cell culture, >=99.5%	1 kg	1,360,700


Antibiotics
수만 편의 논문 인용 횟수
Cell culture 검증 완료
Antibiotic Antimycotic Solution : Penicillin, Streptomycin, Amphotericin B 복합

Cat.No.	Description	Unit	List Price (원)
P4333-100ML	Penicillin-Streptomycin	100 mL	62,400
K0254-20ML	Kanamycin Solution from Streptomyces Kanamyceticus	20 mL	153,000
G1397-10ML	Gentamicin Solution	10 mL	172,600
M4287-2MG	Mitomycin C from Streptomyces Caespitosus	2 mg	327,500
P8833-10MG	Puromycin Dihydrochloride from Streptomyces Alboniger	10 mg	120,800
A5955-100ML	Antibiotic Antimycotic Solution (100X), Stabilized	100 mL	100,400
A0166-25G	Ampicillin sodium salt, BioReagent, suitable for cell culture	25 g	620,500
C1389-5G	Carbenicillin disodium salt, 89.0-100.5% anhydrous basis	5 g	843,000
C0378-25G	Chloramphenicol, >=98% (HPLC)	25 g	150,200


Growth Factor
광범위한 종류의 growth factor
다양한 Origin
Animal component free 제품

Cat.No.	Description	Unit	List Price (원)
E9644-.5MG	hEGF EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	0.5 mg	2,255,600
F0291-25UG	hBFGF FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture	25 μg	633,600
GF003AF-100UG	Fibroblast Growth Factor Basic, Human Recombinant, Animal-free	100 μg	903,500
E2759-15MG	Endothelial Cell Growth Supplement From Bovine Neural Tissue ECGS, suitable for cell culture	15 mg	225,800
I4269-5UG	Interleukin-4 Human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	5 μg	596,000
V7259-10UG	Vascular Endothelial Growth Factor Human, VEGF, Recombinant, expressed in E. coli, powder, suitable for cell culture	10 μg	769,800




Supplements

Cat.No.	Description	Unit	List Price (원)
G8541-100ML	Ala-Gln 200* mM, solution, sterile-filtered, Biotechnology Performance Certified *Alanyl-Glutamine : 수용액 상태에서 불안정 필수 아미노산인 L-glutamine의 안정화된 형태	100 mL	91,500
G7513-100ML	L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture	100 mL	74,000
I9278-5ML	Insulin solution human, sterile-filtered, BioXtra, suitable for cell culture	5 mL	457,900
I0516-5ML	Insulin solution from bovine pancreas, 10 mg/mL insulin in 25 mM HEPES, pH 8.2, BioReagent, sterile-filtered, suitable for cell culture	5 mL	179,100
I3146-5ML	ITS Liquid Media Supplement (100X), liquid, sterile-filtered, BioReagent, suitable for cell culture	5 mL	85,000
M6895-100ML	MEM Vitamin Solution (100X), sterile-filtered, BioReagent, suitable for cell culture	100 mL	51,400
S8636-100ML	Sodium pyruvate solution, 100 mM, sterile-filtered, BioReagent, suitable for cell culture	100 mL	27,700


3D Cell Culture - ECM (Extracellular Matrix)
Mouse EHS
세포가 3D 환경에서 자랄 수 있도록 지지대 역할
일관적인 품질, 안정적 공급

Cat.No.	Description	Unit	List Price (원)
E1270-10ML	ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, liquid, BioReagent, suitable for cell culture	10 mL	985,700
E6909-10ML	ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth-factor reduced, without phenol red, liquid, BioReagent, suitable for cell culture	10 mL	1,165,100
CC131-5ML	Stem Cell Qualified ECM Gel, Stem cell qualified basement membrane extract (BME) for human ES and iPS cell culture	5 mL	742,400
E0282-1ML	MaxGel™ ECM human	1 mL	149,600



3D Cell Culture
TrueGel3D™ HTS : 96 well plate pre-coated hydrogel로 3D 환경에서 drug screening (HTS)
TrueGel3D™ Hydrogel Kits : Hydrogel 기반 다양한 조건에서 3D cell culture kit (Cell recovery, migration, RDG peptide)

Cat.No.	Description	Unit	List Price (원)
TRUE1-1KT	TrueGel3D™ Hydrogel KitsCD cell-degradable crosslinker and RGD peptide	1 kit	562,900
TRUE2-1KT	TrueGel3D™ Hydrogel Kits FAST-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker	1 kit	167,900
TRUE3-1KT	TrueGel3D™ Hydrogel Kits FAST-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker	1 kit	387,900
TRUE4-1KT	TrueGel3D™ Hydrogel Kits FAST-PVA, CD cell-degradable crosslinker	1 kit	393,200
TRUE5-1KT	TrueGel3D™ Hydrogel Kits FAST-PVA, PEG non cell-degradable crosslinker	1 kit	150,800
TRUE6-1KT	TrueGel3D™ Hydrogel Kits SLO-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker	1 kit	166,700
TRUE7-1KT	TrueGel3D™ Hydrogel Kits SLO-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker	1 kit	384,400
TRUE8-1KT	TrueGel3D™ Hydrogel Kits SLO-PVA, CD cell-degradable crosslinker	1 kit	391,300
TRUE9-1KT	TrueGel3D™ Hydrogel Kits SLO-PVA, PEG non cell-degradable crosslinker	1 kit	146,900
TRUE-HTS10	TrueGel3D™ HTS Plate 10 Plates 1	1pck (10 plates)	7,521,200
TRUE-HTS1	TrueGel3D™ HTS Plate 1 Plate	1plate	544,600


Millicell^® HY Multilayer Culture Flask
TC(Tissue culture) treated surface
HY Flask T-1000 : 표면적 1000 cm² (100 Ø dish 약 18개 면적)
HY Flask T-600 : 표면적 600 cm² (100 Ø dish 약 10~11개 면적)

Cat.No.	Description	Unit	List Price (원)
PFHYS1008	Millicell HY Flask T-1000 Sterile 8/pk	1 pack	1,061,900
PFHYS0616	Millicell HY Flask T-600 Sterile 16/pk	1 pack	839,200


Dish, Plate, Pipette
Corning® Erlenmeyer, TC treated, CellSTACK®, ULA plate
Corning® serological pipettes, centrifuge tubes
안정적 공급, 합리적 가격

Cat.No.	Description	Unit	List Price (원)
CLS430488-500EA	Corning^® cryogenic vials, internal thread, 2 mL	500 ea	237,309
CLS431147-25EA	Corning^® Erlenmeyer cell culture flasks	25 ea	470,070
CLS430167-500EA	Corning^® tissue-culture treated culture dishes	500 ea	251,769
CLS3595-50EA	Corning^® 96 Well TC-Treated Microplates	50 ea	104,668
CLS3516-50EA	Corning^® Costar^® TC-Treated Multiple Well Plates, 6 Well	50 ea	97,902
CLS3271-6EA	Corning^® CellSTACK^® cell culture chambers	6 ea	999,166
CLS4520-50EA	Corning^® 96 Well Plate, ULA, Round Bottom	50 ea	1,960,705
CLS430791-500EA	Corning^® 15 mL centrifuge tubes	500 ea	114,301
CLS430828-500EA	Corning^® 50 mL centrifuge tubes	500 ea	189,985
CLS4612-500EA	Corning^® microplate sealing tape, non-sterile	500 ea	255,152
CLS3570-50EA	Corning^® 384 well microplate, low flange	50 ea	367,255
CLS3540-50EA	Corning^® 384 well microplate, low volume, black	50 ea	706,625
CLS4490-100EA	Corning^® Costar^® Stripette^® serological pipettes, individually paper/plastic wrapped, 50 mL	100 ea	167,788
CLS4488-200EA	Corning^® Costar^® Stripette^® serological pipettes, individually paper/plastic wrapped, 10 mL	200 ea	70,662
CLS4489-200EA	Corning^® Costar^® Stripette^® serological pipettes, individually paper/plastic wrapped, 25 mL	200 ea	120,314
CLS4487-200EA	Corning^® Costar^® Stripette^® serological pipettes, individually paper/plastic wrapped, 5 mL	200 ea	69,865


Millicell^® Hanging Inserts
3D Cell culture 환경 구현
Cell differentiation
다양한 Application

Cat.No.	Description	Unit	List Price (원)
PTEP06H48	Millicell^® 8 μm PET HANG, 6WELL 48 PK	48 PK	515,200
PTEP12H48	Millicell^® 8 μm PET HANG, 12WELL 48 PK	48 PK	486,000
PTEP24H48	Millicell^® 8 μm PET HANG, 24WELL 48 PK	48 PK	441,500
PTHT06H48	Millicell^® 0.4 μm PET HANG, 6WELL 48 PK	48 PK	515,200
PTHT12H48	Millicell^® 0.4 μm PET HANG, 12WELL 48 PK	48 PK	486,000
PTHT24H48	Millicell^® 0.4 μm PET HANG, 24WELL 48 PK	48 PK	401,700
PTMP06H48	Millicell^® 5 μm PET HANG, 6WELL 48 PK	48 PK	515,200
PTMP12H48	Millicell^® 5 μm PET HANG, 12WELL 48 PK	48 PK	441,500
PTMP24H48	Millicell^® 5 μm PET HANG, 24WELL 48 PK	48 PK	454,200
PTRP06H48	Millicell^® 1 μm PET HANG, 6WELL 48 PK	48 PK	515,200
PTRP12H48	Millicell^® 1 μm PET HANG, 12WELL 48 PK	48 PK	441,500
PTRP24H48	Millicell^® 1 μm PET HANG, 24WELL 48 PK	48 PK	454,200
PTSP06H48	Millicell^® 3 μm PET HANG, 6WELL 48 PK	48 PK	515,200
PTSP12H48	Millicell^® 3 μm PET HANG, 12WELL 48 PK	48 PK	486,000
PTSP24H48	Millicell^® 3 μm PET HANG, 24WELL 48 PK	48 PK	454,200

* 관련문의: Tel. 031-728-3291 E-mail. dawinbio@dawinbio.com

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