- dCas9-VPR이 안정적으로 발현하는 세포주를 이용해 CRISPRmod, CRISPRa를 편리하게 진행하실 수 있습니다!
- HCT-116, K-562, HAP1, U2OS 세포주 중 선택하신 다음 single guide RNA나 synthetic crRNA complexed with tracrRNA와 함께 transfection하여 유전자 편집을 진행하실 수 있습니다!
제품 정보
| Background cell line | Cat No. | Basal Medium | Supplements |
| HCT-116 | HD dCas9-VPR-002 | RPMI 1640 | 10% FBS, 1% Pen/Strep |
| K-562 | HD dCas9-VPR-005 | IMDM | 10% FBS, 1% Pen/Strep |
| HAP1 | HD dCas9-VPR-011 | IMDM | 10% FBS, 1% Pen/Strep |
| U2OS | HD dCas9-VPR-012 | DMEM, high glucose | 10% FBS, 1% Sodium Pyruvate, 1% Pen/Strep |
제품 자세히 보기
Cas9 stable cell line 실험 결과
| Adherent dCas9-VPR Stable Cell Lines were plated at 10,000 cells/well and reverse transfected using DharmaFECT1 or DharmaFECT4 Transfection Reagent with a pool of synthetic crRNA:tracrRNAs(25 nM) targeting human TTN. Cells were harvested 72 hours post-transfection and the relative gene expression was measured using RT-qPCR. The relative fold transcriptional activation was calculated with the Cq method using GAPDH as the housekeeping gene and normalized to a non-targeting control. Suspension K562 dCas9-VPR Stable Cell Line was electroporated using 100,000 cells/well, and Lonza buffer SF with a pool of synthetic crRNA:tracrRNAs(25 nM) targeting human TTN. Cells were harvested 72 hours post-transfection and the relative gene expression was measured using RT-qPCR. The relative fold transcriptional activation for was calculated with the Cq method using GAPDH as the housekeeping gene and normalized to a non-targeting control.
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제품 문의 Tel. 031-728-3233, 3239 E-mail. technical@dawinbio.com
#Horizon discovery #다윈바이오 #dCas9-VPR #CRISPRmod #CRISPRa
- dCas9-VPR이 안정적으로 발현하는 세포주를 이용해 CRISPRmod, CRISPRa를 편리하게 진행하실 수 있습니다!
- HCT-116, K-562, HAP1, U2OS 세포주 중 선택하신 다음 single guide RNA나 synthetic crRNA complexed with tracrRNA와 함께 transfection하여 유전자 편집을 진행하실 수 있습니다!
제품 정보
제품 자세히 보기
Cas9 stable cell line 실험 결과
Adherent dCas9-VPR Stable Cell Lines were plated at 10,000 cells/well and reverse transfected using DharmaFECT1 or DharmaFECT4 Transfection Reagent with a pool of synthetic crRNA:tracrRNAs(25 nM) targeting human TTN. Cells were harvested 72 hours post-transfection and the relative gene expression was measured using RT-qPCR. The relative fold transcriptional activation was calculated with the Cq method using GAPDH as the housekeeping gene and normalized to a non-targeting control.
Suspension K562 dCas9-VPR Stable Cell Line was electroporated using 100,000 cells/well, and Lonza buffer SF with a pool of synthetic crRNA:tracrRNAs(25 nM) targeting human TTN. Cells were harvested 72 hours post-transfection and the relative gene expression was measured using RT-qPCR. The relative fold transcriptional activation for was calculated with the Cq method using GAPDH as the housekeeping gene and normalized to a non-targeting control.
제품 문의 Tel. 031-728-3233, 3239 E-mail. technical@dawinbio.com
#Horizon discovery #다윈바이오 #dCas9-VPR #CRISPRmod #CRISPRa