- Cas9이 안정적으로 발현하는 세포주를 이용해 유전자 편집을 편리하게 진행하실 수 있습니다!
- HAP1, A549, Jurkat 등 인기 있는 세포주를 선택하신 다음 CRISPR guide RNA만 넣어 간편하게 유전자 KO을 하실 수 있습니다!
제품 정보
| Cell line background | Cat No. | Basal Medium | Supplements |
| A549 | HD Cas9-001 | RPMI 1640 | 10% FBS, 1% Pen/Strep |
| HCT-116 | HD Cas9-002 | RPMI 1640 | 10% FBS, 1% Pen/Strep |
| Jurkat | HD Cas9-004 | RPMI 1640 | 10% FBS, 1% Pen/Strep |
| K-562 | HD Cas9-005 | IMDM | 10% FBS, 1% Pen/Strep |
| RKO | HD Cas9-007 | RPMI 1640 | 10% FBS, 1% Pen/Strep |
| NIH/3T3 | HD Cas9-010 | DMEM, high glucose, GlutaMAX | 10% FBS, 1% Pen/Strep |
| HAP1 | HD Cas9-011 | IMDM | 10% FBS, 1% Pen/Strep |
| U2OS | HD Cas9-012 | DMEM, high glucose | 10% FBS, 1% Sodium Pyruvate, 1% Pen/Strep |
제품 자세히 보기
Cas9 stable cell line 실험 결과
| Adherent Cas9 Stable Cell Lines were plated at 10,000 cells/well and reverse transfected using DharmaFECT1 or DharmaFECT4 Transfection Reagent with synthetic sgRNA (25 nM) targeting PPIB. Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared to a non-targeting control.
Suspension Cas9 Stable Cell Lines were electroporated using 100,000 cells/well, and Lonza buffer SE or SF, respectively, with synthetic sgRNA (5 µM) targeting PPIB. Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared ion to a non-targeting control.
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제품 문의 Tel. 031-728-3233, 3239 E-mail. technical@dawinbio.com
#Horizon discovery #다윈바이오 #Cas9 stable cell line #KO DIY
- Cas9이 안정적으로 발현하는 세포주를 이용해 유전자 편집을 편리하게 진행하실 수 있습니다!
- HAP1, A549, Jurkat 등 인기 있는 세포주를 선택하신 다음 CRISPR guide RNA만 넣어 간편하게 유전자 KO을 하실 수 있습니다!
제품 정보
제품 자세히 보기
Cas9 stable cell line 실험 결과
Adherent Cas9 Stable Cell Lines were plated at 10,000 cells/well and reverse transfected using DharmaFECT1 or DharmaFECT4 Transfection Reagent with synthetic sgRNA (25 nM) targeting PPIB. Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared to a non-targeting control.
Suspension Cas9 Stable Cell Lines were electroporated using 100,000 cells/well, and Lonza buffer SE or SF, respectively, with synthetic sgRNA (5 µM) targeting PPIB. Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared ion to a non-targeting control.
제품 문의 Tel. 031-728-3233, 3239 E-mail. technical@dawinbio.com
#Horizon discovery #다윈바이오 #Cas9 stable cell line #KO DIY