Exosome 관련Exosome Isolation을 위한 항체

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Antibody for Exosome Isolation


Exosomes are cell-derived vesicles formed by a lipid bilayer membrane, and its diameter is 30-100 nm. Exosomesare observed in body fluid, such as saliva, blood, urine, amniotic fluid, malignant ascites, and are secreted fromcultured cells. Recently, it has been shown that exosomes include various proteins and RNAs, which have apossibility to function in intercellular signal transduction. These products are the antibodies which can specificallydetect CD9, CD63 and CD81 known as exosome markers. These antibodies are suitable for isolation of exosome byimmunoprecipitation. ExoTrapTM Spin Columns utilize immobilized CD9 antibody to capture exosomes frombiological fluids in as little as 30 minutes. The contents of the captured exosomes are then readily processed fordownstream analysis by western blotting, mass spectrometry, PCR, or sequencing, etc


Figure. Generic exosome shown withmolecules commonly foundassociated with exosomes. Notall molecules will be found withany given exosome and othermolecules may also be found.


Feature                                                          Advantages

monoclonal                                                         High specificity for exosome membrane proteins CD9, CD63, CD81         

human only                                                         Nearly 100% of exosomes in a 150 µL sample can be isolated by using only 1 µg of antibody

efficient                                                                       Supported samples: CD9 - serum  , CD63, CD81- serum, culture supernatant 

not cross reactive                                             Useful for analyses of exosome surface antigen proteins, endogenous RNA (miRNA) andproteins

with other tetraspanin


Feature  and Advantages

■ High purity exosome can be isolated from serum, plasma, urine, saliva and cell culturesupernatant within 30 minutes (All human samples has been tested).

 Spin column type which is easy to use



Anti CD9 for Exosome Isolation
 

CD9 is a cell surface glycoprotein which belongs to the tetraspanin superfamily. CD9 is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation.  


Anti CD63 for Exosome Isolation 

CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a cell surface glycoprotein which belongs to the tetraspanin superfamily. CD63 is known to complex with integrins. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells.


Anti CD81 for Exosome Isolation 

CD81 (TAPA-1) is a cell surface protein which belongs to the tetraspanin superfamily. CD81 is identified as a component of the B lymphocyte receptor (BCR) and as a receptor for the Hepatitis C Virus.


ExoTrap Exosome Isolation Spin Column Kit for Protein Research 

Exosomes are 40 ‒100 nm membrane vesicles. They are found in many biological fluids including blood, urine, saliva, amniotic fluid, cell culture supernatants, and malignant ascites fluids. CD9 is a well known surface marker for exosomes. ExoTrap Spin Columns utilize immobilized CD9 antibody to capture exosomes from biological fluids in as little as 30 minutes. The contents of the captured exosomes are then readily processed for downstream analysis by western blotting, mass spectrometry, PCR, or sequencing, etc.


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Description
Cat. No.
Size
Anti CD9, Human (Mouse) Unlabeled, 12A12
CAC-SHI-EXO-M01
100UL
Anti CD63, Human (Mouse) Unlabeled, 8A12
CAC-SHI-EXO-M02
100UL
Anti CD81, Human (Mouse) Unlabeled, 12C4
CAC-SHI-EXO-M03
100UL
Anti CD9, Human (Mouse) Unlabeled, 12A12
CAC-SHI-EXO-M01-50
50UL
Anti CD63, Human (Mouse) Unlabeled, 8A12
CAC-SHI-EXO-M02-50
50UL
Anti CD81, Human (Mouse) Unlabeled, 12C4
CAC-SHI-EXO-M03-50
50UL
Anti CD9, Human (Mouse) Biotin, 12A12
CAC-SHI-EXO-M01-B
100UL
Anti CD63, Human (Mouse) Biotin, 8A12
CAC-SHI-EXO-M02-B
100UL
Anti CD81, Human (Mouse) Biotin, 12C4
CAC-SHI-EXO-M03-B
100UL
Anti CD9, Human (Mouse) Label(Tide FluorTM5), 12A12
CAC-SHI-EXO-M01-TF5
100UL
Anti CD63, Human (Mouse) Label(Tide FluorTM5), 8A12
CAC-SHI-EXO-M02-TF5
100UL
Anti CD81, Human (Mouse) Label(Tide FluorTM5), 12C4
CAC-SHI-EXO-M03-TF5
100UL
ExoTrap Exosome Isolation Spin Column
CSR-SHI-EXO-K010
10PREP
CD9/CD63 Exosome ELISA Kit, Human
CSR-EXH0102EL
1KIT


학술 문의    T. 031-728-3233    E. technical@dawinbio.com

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