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ToolsWestern Blot 관련 제품 안내

Abcam
조회수 14768

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Western Blot 실험을 위한 다양한 시약


* Cat no.를 클릭하시면 제품 상세페이지를 확인하실 수 있습니다.

Lysis Buffers

ProductCat no.Size 
10X RIPA Bufferab15603415 ml
Native lysis Buffer ab15603515 ml
10x Red Blood Cell (RBC) Lysis Buffer ab204733100 ml


Cell fractionation

ProductCat no.Size 
* Cell Fractionation Kit - Standardab1097191 kit
Nuclear Extraction Kitab113474100 tests
Mitochondria/Cytosol Fractionation Kitab6532025, 100 tests
Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)ab1101711 kit
Mitochondria Isolation Kit for Cultured Cells (without Dounce Homogenizer)ab1101701 kit
Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer)ab1101691 kit
Mitochondria Isolation Kit for Tissue (without Dounce Homogenizer)ab1101681 kit
Plasma Membrane Protein Extraction Kit ab6540050 tests

* Cell sample에서 cytosolic, mitochondrial, nuclear fraction을 각각 농축할 수 있는 제품입니다. (단, 각 fraction이 완벽히 분리되어 정제되는 것은 아닙니다.)


PIC

ProductCat no.Size 
Protease Inhibitor Cocktail (EDTA가 별도의 vial로 제공)ab2713063, 10, 30 ml
Protease Inhibitor Cocktail EDTA Freeab2700553, 10, 30 ml
Protease and Phosphatase Inhibitor Cocktail (EDTA가 별도의 vial로 제공)ab2011191 kit
Protease and Phosphatase Inhibitor Cocktail (EDTA-free) ab2011201 ml

* EDTA: Inhibits metalloproteases by chelating divalent metal ions


BCA or Bradford

ProductCat no.Size 
BCA Protein Assay Kit (0.5 µg - 30 µg)ab1025361000 tests
BCA protein assay kit reducing agent compatible (microplate) ab2070031000 tests
Bradford Assay Kit (with standard), 5Xab1025351000 tests


SDS-PAGE related products

ProductCat no.Size 
* Western Blot Stripping Buffer ab282569500 ml
* InstantBlue Coomassie Protein Stainab1192111000 ml
10X Blocking Buffer ab12658750 ml
Tween 20 (Polyoxyethelenesorbitan Monolaurate)ab128987125 ml
25x PBS Buffer pH 7.6ab64026125, 1000 ml
20x PBS buffer with Tween 20ab64247125, 1000 ml
25x TBS (pH 7.4)ab64248125, 1000 ml
20x TBS-T with Tween 20ab64204125, 1000 ml

* 상시 국내 재고를 보유하고 있는 품목입니다.


- InstantBlue Coomassie Protein Stain (ab119211) 의 간편한 protocol


Protein markers

ProductCat no.Size 
Western Blot Marker Penab1668581 unit
* Prestained Protein Ladder – Mid-range molecular weight (10-180 kDa)ab116027500 µl
* Prestained Protein Ladder – Broad molecular weight (10-245 kDa)ab116028500 µl
* Prestained Protein Ladder – Extra broad molecular weight (5-245 kDa)ab116029500 µl
Prestained Protein Ladder – Extra broad molecular weight (6.5-270 kDa)ab234592500 µl
Unstained Protein Ladder (10-200 kDa) ab234618500 µl

* 상시 국내 재고를 보유하고 있는 품목입니다.


Prestained Protein Ladder – Mid-range molecular weight (10-175 kDa) (ab115832)Prestained Protein Ladder – Mid-range molecular weight (10-180 kDa) (ab116027)


Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)Prestained Protein Ladder – Extra broad molecular weight (5-245 kDa) (ab116029)
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Prestained Protein Ladder – Extra broad molecular weight (6.5-270 kDa) (ab234592)Unstained Protein Ladder (10-200 kDa) (ab234618)


Membrane

ProductCat no.Size 
Nitrocellulose Transfer Membrane- 0.45 µm ab13341210 sheets 
Nitrocellulose Transfer Membrane- 0.22 µm  ab13341310 sheets 
Low Fluorescence Western Membrane, PVDFab13341110 sheets 

* Pre-cut membranes (ab133412, ab133413  8 x 10cm, ab133411  7 x 9 cm), compatible with gels 10 x 10 cm and smaller.  


Protein bead (for IP)

ProductCat no.Size 
Protein A Magnetic Beadsab2142861 ml
Protein A/G Sepharose®ab1932621, 5, 25, 100 ml
Protein A Sepharose®ab1932561, 5, 25, 100 ml
Protein G Sepharose®ab1932591, 5, 25, 100 ml
Protein G Agarose (High Affinity)ab1932581, 5, 25, 100 ml
Protein A Agaroseab1932541, 5, 25, 100 ml
Protein A Agarose (High Affinity)ab1932551, 5, 25, 100 ml


Secondary antibody

ProductApplicationCat no.Size 
* Goat Anti-Rabbit IgG H＆L (HRP)WB, IHC-P, ELISA, IPab205718500 ug
* Goat Anti-Rabbit IgG H&L (HRP)WB, IHC-P, IHC-Fr, ELISA, ICC, Immunomicroscopy, Dot blotab67211 mg 
* Goat Anti-Rabbit IgG H&L (HRP)WB, IHC-P, ELISA, ICCab970511 mg 
* Rabbit Anti-Mouse IgG H&L (HRP)WB, IHC-P, IHC-Fr, ELISA, ICC/IF, Immunomicroscopy, Dot blotab67281 mg 
* Goat Anti-Mouse IgG H&L (HRP)WB, IHC-P, IHC-Fr, ICC, IP, ELISA, Immunomicroscopy, Dot blotab67891 mg 
* Goat Anti-Mouse IgG H&L (HRP)WB, IHC-P, ICC, ELISAab970231 mg 
* Goat Anti-Mouse IgG H&L (HRP)WB, IHC-P, IP, ELISAab205719500 ug
* Rabbit Anti-Goat IgG H&L (HRP)WB, IHC-P, IHC-Fr, ELISA, ICC/IF, Immunomicroscopy, Dot blotab67411 mg 

* 상시 국내 재고를 보유하고 있는 품목입니다.


IP 전용 Secondary antibody

- Native 상태의 primary antibody 만을 인식

- WB 과정에서 denatured primary antibody의 heavy/light chain 인식을 현저히 감소시킴

- Target protein이 30 kDa 이하일 때 효과적

ProductCat no.Size 
* VeriBlot for IP Detection Reagent (HRP)
  (Rabbit total IgG, 그 외 reactivity는 datasheet 참고)		ab131366500 ul 
Mouse anti-goat IgG for IP (HRP) ab157532100 ul 
* Anti-mouse IgG for IP (HRP) ab131368100 ul 
Anti-mouse IgG for IP (Biotin) ab13136750 ug

* 상시 국내 재고를 보유하고 있는 품목입니다.


IP 용 light chain specific antibody 

- Antibody의 light chain 만을 인식, Target protein이 30 kDa 이상일 때 효과적

ProductCat no.Size 
Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697500 ul 
Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP) ab99811500 ul 
Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)ab99696500 ul 
Mouse monoclonal Anti-Rat kappa light chain antibody [KT102] (HRP) ab170326100 ul 


기타

ProductCat no.Size 
* 10kD Spin Column ab9334925 units

* 상시 국내 재고를 보유하고 있는 품목입니다.

 


WB 실험 protocol 보기                    WB 샘플 준비 protocol 보기 



*학술 문의 : Tel. 031-728-3234, 3237   E-mail. technical@dawinbio.com   카카오톡 플러스친구 ID: abcam

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IHC(ImmunoHistoChemistry) 관련 제품 High-purity Bioactive Proteins
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