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ToolsAntibody Conjugation Kit

Abcam
조회수 13453

 

빠르고 쉬운 abcam의

Antibody Conjugation


  • 빠르고 쉬운 conjugation 프로토콜
    - 1) 정제된 Primary antibody 2) Modifier 추가 3) Conjugation Mix 추가 4) Quencher 추가 5) 완료!
    - 30초 정도의 hands on time
    - 20분 또는 4시간 안에 conjugation 반응 완료
  • Antibody가 손실되지 않음  별도의 purification 또는 separation 과정이 없음
  • 다양한 label 가능 fluorochromes, enzyme, biotin/streptavidin, oligonucleotides, nanoparticles 등
  • Conjugated antibody는 바로 다양한 application에 활용 가능 flow cytometry, ICC/IF, IHC, WB, ELISA 등


잠깐! Antibody conjugation을 위해 필요한 조건을 확인해 주세요.

v  정제된 antibody만 conjugation에 적합합니다. (crude serum이나 tissue culture supernatant의 경우 부적합)

v  Antibody에 BSA, glycine, tris, azide 등이 포함되어 있을 경우 conjugation 반응에 방해가 되므로 제거가 필요합니다.

v  Antibody 농도는 최소 0.5 mg/ml 이상 이어야 합니다.


Fluorescent / Fast type

* 아래 제품은 모두 Fast type 입니다.

   Fast type: 20분 안에 antibody conjugation을 완료

Cat no.ProductEx/Em (nm)Size
ab236553Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link®495/519 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab269820Alexa Fluor® 555 Conjugation Kit (Fast) - Lightning-Link®555/565 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab269821Alexa Fluor® 568 Conjugation Kit (Fast) - Lightning-Link®578/603 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab269822Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link®590/617 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab269823Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®652/668 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab269824Alexa Fluor® 700 Conjugation Kit (Fast) - Lightning-Link®730/745 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab195224AMCA Conjugation Kit (Fast) - Lightning-Link®350/445 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab188287Cy3® Conjugation Kit (Fast) - Lightning-Link®548/561 30 µg, 100 µg, 3 x 100 µg, 1 mg
ab188288Cy5® Conjugation Kit (Fast) - Lightning-Link®647/665 3 x 10 µg, 100 µg, 300 µg, 1 mg
ab201797DyLight® 350 Conjugation Kit (Fast) - Lightning-Link®353/432 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab201798DyLight® 405 Conjugation Kit (Fast) - Lightning-Link®400/420 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab201799DyLight® 488 Conjugation Kit (Fast) - Lightning-Link®493/518 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab201800DyLight® 550 Conjugation Kit (Fast) - Lightning-Link®562/576 3 x 10 µg, 100 µg, 300 µg, 1 mg
ab201801DyLight® 594 Conjugation Kit (Fast) - Lightning-Link®593/618 30 µg, 100 µg, 3 x 100 µg, 1 mg
ab201802DyLight® 633 Conjugation Kit (Fast) - Lightning-Link®638/658 30 µg, 100 µg, 300 µg, 1 mg
ab201803DyLight® 650 Conjugation Kit (Fast) - Lightning-Link®652/672 30 µg, 100 µg, 3 x 100 µg, 1 mg
ab201804DyLight® 680 Conjugation Kit (Fast) - Lightning-Link®692/712 30 µg, 100 µg, 3 x 100 µg, 1 mg
ab201805DyLight® 755 Conjugation Kit (Fast) - Lightning-Link®754/776 30 µg, 100 µg, 3 x 100 µg, 1 mg
ab201806DyLight® 800 Conjugation Kit (Fast) - Lightning-Link®777/794 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab188285FITC Conjugation Kit (Fast) - Lightning-Link®495/5193 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab269904FluProbes 647H Conjugation Kit - Lightning-Link®653/674 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab188286Rhodamine Conjugation Kit (Fast) - Lightning-Link®570/590 3 x 10 µg, 100 µg, 300 µg, 1 mg
ab195225Texas Red® Conjugation Kit (Fast) - Lightning-Link®595/613 30 µg, 100 µg, 3 x 100 µg, 1 mg
ab269895Atto 390 Conjugation Kit (Fast) - Lightning-Link®388/468 3 x 10 µg, 100 µg, 3 x 100 µg
ab269896Atto 488 Conjugation Kit (Fast) - Lightning-Link®504/530 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab269897Atto 565 Conjugation Kit (Fast) - Lightning-Link®570/598 3 x 10 µg, 100 µg, 3 x 100 µg
ab269898Atto 633 Conjugation Kit (Fast) - Lightning-Link®634/660 3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg

 * Atto dye : 특정 파장대의 빛을 흡수한 다음 높은 형광을 방출합니다.


Fluorescent / Standard type

* Standard type: 4시간 안에 antibody conjugation을 완료할 수 있는 제품입니다.

Cat no.ProductEx/Em (nm)Size
ab201807APC Conjugation Kit - Lightning-Link®650/6603 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab102855APC/Cy5.5® Conjugation Kit - Lightning-Link®650/6903 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab102859APC/Cy7® Conjugation Kit - Lightning-Link®652/7903 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab269891Cy5.5 Conjugation Kit - Lightning-Link®678/7033 x 10 µg, 100 µg, 3 x 100 µg
ab102884FITC Conjugation Kit - Lightning-Link®495/51930 µg, 100 µg, 300 µg, 1 mg
ab102871B-Phycoerythrin Conjugation Kit - Lightning-Link®545, 565/57560 µg, 3 x 60 µg, 600 µg
ab102918PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®496, 546/5783 x 10 µg, 60 µg, 3 x 60 µg, 600 µg, 5 x 600 µg
ab269900PE/Atto 594 Conjugation Kit - Lightning-Link®535/6273 x 10 µg, 60 µg, 3 x 60 µg, 600 µg
ab102893PE/Cy5® Conjugation Kit - Lightning-Link®565/6663 x 10 µg, 60 µg, 3 x 60 µg, 600 µg
ab102899PE/Cy5.5® Conjugation Kit - Lightning-Link®496, 565/6703 x 10 µg, 60 µg, 3 x 60 µg, 600 µg
ab102903PE/Cy7® Conjugation Kit - Lightning-Link®565/77830 µg, 60 µg, 3 x 60 µg, 600 µg
ab269899PE/Texas Red Conjugation Kit - Lightning-Link®496/6153 x 10 µg, 60 µg, 3 x 60 µg, 600 µg
ab102907PerCP Conjugation Kit - Lightning-Link®482/6783 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab102911PerCP/Cy5.5® Conjugation Kit - Lightning-Link®482/6783 x 10 µg, 100 µg, 3 x 100 µg, 1 mg


Enzyme & Tags

TypeCat no.ProductSize
Enzymeab102850 Alkaline phosphatase Conjugation Kit - Lightning-Link®3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab269901 Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link®3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg
ab102887 Glucose Oxidase Conjugation Kit - Lightning-Link®3 x 10 µg, 3 x 100 µg, 1 mg
ab102890 HRP Conjugation Kit - Lightning-Link®3 x 10 µg, 3 x 100 µg, 1 mg, 1 x 5 mg, others
Biotin/
Streptavidin		ab201795 Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®30 µg, 100 µg, 3 x100 µg, 1 mg
ab201796 Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link®30 µg, 100 µg, 3 x100 µg, 1 mg
ab102921 Streptavidin Conjugation Kit - Lightning-Link®3 x 10 µg, 100 µg, 3 x 100 µg, 1 mg


Others - Europium, Latex, Magnetic

Type
Cat no.
Product
Size
Europiumab269889Europium Conjugation Kit10 x 4 μg, 1 x 40 μg, 16 μg
Latexab269894Latex Conjugation Kit – 400nm Black4 x 4 μg, 1 x 40 μg, 40 μg
ab269892Latex Conjugation Kit – 400nm Blue10 x 4 μg, 1 x 40 μg, 16 μg
ab269893Latex Conjugation Kit – 400nm Red10 x 4 μg, 1 x 40 μg, 16 μg
Magneticab269890Magnetic Conjugation Kit1 x 200 μg, 60 μg, 200 μg







              ab269889  Europium Conjugation Kit

              Typical results obtained from fluorescence reader (bottom), 

              showing europium conjugated to a monoclonal anti-CRP antibody.

             The conjugate is easily able to detect as little as 63pg/mL CRP, in 

             part due to the low background and lack of aggregation. 


Oligonucleotide

Cat no.
Product
Size
Oligonucleotide Conjugation Kit1, 3 tests


Gold

Cat no.ProductSize
ab201808GOLD Conjugation Kit (10nm, 20 OD)3, 10 tests, 1 x 25 µg
ab269903GOLD Conjugation Kit (10nm, Maleimide)3 x 5 µg, 400 µl
ab188215GOLD Conjugation Kit (20nm, 20 OD)1, 3, 10 tests
ab269902GOLD Conjugation Kit (20nm, Maleimide)120 µl, 1 x 50 µg
ab154873GOLD Conjugation Kit (40nm, 20 OD)1, 3, 10 tests
ab154876GOLD Conjugation Kit (80nm, 20 OD)1, 10 tests, 3 x 0.5 µg
ab188290GOLD-Oligonucleotide Conjugation Kit3 x 2.5 µg, 1 x 25 µg
ab186922GOLD-Biotin Conjugation Reagent (40nm, 10 OD)200 μl, 1 ml
ab186864GOLD-Streptavidin Conjugation Reagent (40nm, 10 OD)200 μl, 1 ml






                             ab201808  GOLD Conjugation Kit (10nm, 20OD)



Metal

Cat no.ProductSize
ab269826  141Pr Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269827  142Nd Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269828  143Nd Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269829  144Nd Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269830  146Nd Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269831  147Sm Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269832  148Nd Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269833  149Sm Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269834  151Eu Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269835  152Sm Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269836  153Eu Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269837  154Sm Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269838  156Gd Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269839  158Gd Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269840  159Tb Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269841  160Gd Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269842  162Dy Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269843  164Dy Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269844  165Ho Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269845  166Er Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269846  167Er Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269847  168Er Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269848  169Tm Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269849  170Er Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269850  171Yb Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269851  172Yb Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269852  174Yb Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269853  175Lu Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg
ab269854  176Yb Metal Conjugation Kit - Lightning-Link®10 μg, 100 μg



* 제품 규격 별로 권장하는 antibody의 용량과 사용 횟수가 다릅니다. 보다 자세한 내용은 datasheet를 참고해주세요.


Conjugation을 위한 antibody Purification & Concentration kit 정보 보기



*학술 문의 : Tel. 031-728-3234, 3237   E-mail. technical@dawinbio.com   카카오톡 플러스친구 ID: abcam

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